Key Responsibilities: Perform aseptic handling for various upstream operations. Prepare media and feeds for shake flask experiments and bioreactor batches. Handle shake flasks for clone selection, media optimization, and feed strategy development. Prepare and maintain bioreactors at different scales (1L, 5L, 20L). Execute upstream batches in small-scale and large-scale bioreactors. Conduct in-process sample analysis including cell count, viability, and metabolite levels (glucose, lactate, ammonia, glutamine, etc.). Draft and maintain Standard Operating Procedures (SOPs) for instruments and processes. Participate in shift duties for continuous batch monitoring and process support. Collaborate effectively within a team environment to ensure smooth upstream operations.